Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression by promoting host mRNA degradation
Identifieur interne : 003D09 ( Main/Exploration ); précédent : 003D08; suivant : 003D10Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression by promoting host mRNA degradation
Auteurs : Wataru Kamitani [États-Unis] ; Krishna Narayanan [États-Unis] ; Cheng Huang [États-Unis] ; Kumari Lokugamage [États-Unis] ; Tetsuro Ikegami [États-Unis] ; Naoto Ito [États-Unis, Japon] ; Hideyuki Kubo [États-Unis] ; Shinji Makino [États-Unis]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2006.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Animaux, Dimérisation, Expression des gènes, Facteur-3 de régulation d'interféron (métabolisme), Gènes rapporteurs (génétique), Humains, Interféron bêta (génétique), Lignée cellulaire, Plasmides (génétique), Protéines virales (génétique), Protéines virales (métabolisme), Stabilité de l'ARN, Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
English descriptors
- KwdEn :
- Animals, Cell Line, Chlorocebus aethiops, Dimerization, Gene Expression, Genes, Reporter (genetics), Humans, Interferon Regulatory Factor-3 (metabolism), Interferon-beta (genetics), Plasmids (genetics), RNA Stability, RNA, Messenger (genetics), SARS Virus (genetics), SARS Virus (metabolism), Transfection, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : Interferon-beta, RNA, Messenger, Viral Proteins.
- chemical , metabolism : Interferon Regulatory Factor-3, Viral Proteins.
- genetics : Genes, Reporter, Plasmids, SARS Virus.
- metabolism : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, Dimerization, Gene Expression, Humans, RNA Stability, Transfection.
Abstract
Severe acute respiratory syndrome (SARS) coronavirus (SCoV) causes a recently emerged human disease associated with pneumonia. The 5′ end two-thirds of the single-stranded positive-sense viral genomic RNA, gene 1, encodes 16 mature proteins. Expression of nsp1, the most N-terminal gene 1 protein, prevented Sendai virus-induced endogenous IFN-β mRNA accumulation without inhibiting dimerization of IFN regulatory factor 3, a protein that is essential for activation of the IFN-β promoter. Furthermore, nsp1 expression promoted degradation of expressed RNA transcripts and host endogenous mRNAs, leading to a strong host protein synthesis inhibition. SCoV replication also promoted degradation of expressed RNA transcripts and host mRNAs, suggesting that nsp1 exerted its mRNA destabilization function in infected cells. In contrast to nsp1-induced mRNA destablization, no degradation of the 28S and 18S rRNAs occurred in either nsp1-expressing cells or SCoV-infected cells. These data suggested that, in infected cells, nsp1 promotes host mRNA degradation and thereby suppresses host gene expression, including proteins involved in host innate immune functions. SCoV nsp1-mediated promotion of host mRNA degradation may play an important role in SCoV pathogenesis.
Url:
DOI: 10.1073/pnas.0603144103
PubMed: 16912115
PubMed Central: 1568942
Affiliations:
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Le document en format XML
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and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Lokugamage, Kumari" sort="Lokugamage, Kumari" uniqKey="Lokugamage K" first="Kumari" last="Lokugamage">Kumari Lokugamage</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Laboratory of Zoonotic Diseases, Division of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Zoonotic Diseases, Division of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu 501-1193</wicri:regionArea><orgName type="university">Université de Gifu</orgName><placeName><settlement type="city">Gifu (ville)</settlement><region type="province">Région du Chūbu</region><region type="prefecture">Préfecture de Gifu</region></placeName></affiliation></author><author><name sortKey="Kubo, Hideyuki" sort="Kubo, Hideyuki" uniqKey="Kubo H" first="Hideyuki" last="Kubo">Hideyuki Kubo</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name><affiliation wicri:level="2"><nlm:aff id="aff1">*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019; and</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>*Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston</wicri:cityArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Dimerization</term><term>Gene Expression</term><term>Genes, Reporter (genetics)</term><term>Humans</term><term>Interferon Regulatory Factor-3 (metabolism)</term><term>Interferon-beta (genetics)</term><term>Plasmids (genetics)</term><term>RNA Stability</term><term>RNA, Messenger (genetics)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Transfection</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Animaux</term><term>Dimérisation</term><term>Expression des gènes</term><term>Facteur-3 de régulation d'interféron (métabolisme)</term><term>Gènes rapporteurs (génétique)</term><term>Humains</term><term>Interféron bêta (génétique)</term><term>Lignée cellulaire</term><term>Plasmides (génétique)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Stabilité de l'ARN</term><term>Transfection</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferon-beta</term><term>RNA, Messenger</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon Regulatory Factor-3</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Reporter</term><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Gènes rapporteurs</term><term>Interféron bêta</term><term>Plasmides</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteur-3 de régulation d'interféron</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Dimerization</term><term>Gene Expression</term><term>Humans</term><term>RNA Stability</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Dimérisation</term><term>Expression des gènes</term><term>Humains</term><term>Lignée cellulaire</term><term>Stabilité de l'ARN</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) coronavirus (SCoV) causes a recently emerged human disease associated with pneumonia. The 5′ end two-thirds of the single-stranded positive-sense viral genomic RNA, gene 1, encodes 16 mature proteins. Expression of nsp1, the most N-terminal gene 1 protein, prevented Sendai virus-induced endogenous IFN-β mRNA accumulation without inhibiting dimerization of IFN regulatory factor 3, a protein that is essential for activation of the IFN-β promoter. Furthermore, nsp1 expression promoted degradation of expressed RNA transcripts and host endogenous mRNAs, leading to a strong host protein synthesis inhibition. SCoV replication also promoted degradation of expressed RNA transcripts and host mRNAs, suggesting that nsp1 exerted its mRNA destabilization function in infected cells. In contrast to nsp1-induced mRNA destablization, no degradation of the 28S and 18S rRNAs occurred in either nsp1-expressing cells or SCoV-infected cells. These data suggested that, in infected cells, nsp1 promotes host mRNA degradation and thereby suppresses host gene expression, including proteins involved in host innate immune functions. SCoV nsp1-mediated promotion of host mRNA degradation may play an important role in SCoV pathogenesis.</p></div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Préfecture de Gifu</li><li>Région du Chūbu</li><li>Texas</li></region><settlement><li>Gifu (ville)</li></settlement><orgName><li>Université de Gifu</li></orgName></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Kamitani, Wataru" sort="Kamitani, Wataru" uniqKey="Kamitani W" first="Wataru" last="Kamitani">Wataru Kamitani</name></region><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><name sortKey="Kubo, Hideyuki" sort="Kubo, Hideyuki" uniqKey="Kubo H" first="Hideyuki" last="Kubo">Hideyuki Kubo</name><name sortKey="Lokugamage, Kumari" sort="Lokugamage, Kumari" uniqKey="Lokugamage K" first="Kumari" last="Lokugamage">Kumari Lokugamage</name><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></country><country name="Japon"><region name="Région du Chūbu"><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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